Extraction, purification of Prostate Specific Antigen (PSA) and establishment of radioimmunoassay system as a diagnostic tool for prostate disorders.

Extraction, purification of Prostate Specific Antigen (PSA) and establishment of radioimmunoassay system as a diagnostic tool for prostate disorders.

J Immunoassay Immunochem. 2017 Nov 16;:

Authors: Abu-Bakr El-Bayoumy AS, Hessien Keshta AT, Sallam KM, Ebeid NH, Elsheikh HM, Bayoumy BE

Abstract
This study aimed to provide an easy and effective method for extraction and purification of prostate specific antigen (PSA) from human seminal fluid with high quantity (14 mg) and high purity (98%). The obtained PSA was injected into rabbits for production of anti-PSA polyclonal antibody (titer 1/1000), labeled with radioactive iodine-125 for preparation of radioactive PSA tracer (purity 98 ± 1.8% and specific activity 64 ± 1.9 µCi/µg), and used in preparation of PSA standards. All prepared components can be used in PSA immunoassays specially radioimmunoassay (RIA) kit preparation as a diagnostic tool for prostatic diseases.

PMID: 29144195 [PubMed - as supplied by publisher]

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