Reply to Comment: ‘A novel method for fast and robust estimation of fluorescence decay dynamics using constrained least-square deconvolution with Laguerre expansion’

In this response we underscore that the instrumentation described in the original publication (Liu et al 2012 Phys. Med. Biol . 57 843–65) was based on pulse-sampling technique, while the comment by Zhang et al is based on the assumption that a time-correlated single photon counting (TCSPC) instrumentation was used. Therefore the arguments made in the comment are not applicable to the noise model reported by Liu et al . As reported in the literature (Lakowicz 2006 Principles of Fluorescence Spectroscopy (New York: Springer)), while in the TCSPC the experimental noise can be estimated from Poisson statistics, such an assumption is not valid for pulse-sampling (transient recording) techniques. To further clarify this aspect, we present here a comprehensive noise model describing the signal and noise propagation of the pulse sampling time-resolved fluorescence detection. Experimental data recorded in various conditions are analyzed as a case ...

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