IJMS, Vol. 18, Pages 2286: Efficient Generation of Genome-Modified Mice Using Campylobacter jejuni-Derived CRISPR/Cas

IJMS, Vol. 18, Pages 2286: Efficient Generation of Genome-Modified Mice Using Campylobacter jejuni-Derived CRISPR/Cas

International Journal of Molecular Sciences doi: 10.3390/ijms18112286

Authors: Wataru Fujii Arisa Ikeda Koji Sugiura Kunihiko Naito

Mammalian zygote-mediated genome-engineering by Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/Cas is currently used for the generation of genome-modified animals. Here, we report that a Campylobacter jejuni-derived orthologous CRISPR/Cas system recognizes a 5′-NNNVRYAC sequence as a protospacer-adjacent motif in mouse zygotes, and is applicable for efficient generation of knockout mice. Moreover, this novel CRISPR/Cas can be used for zygote-mediated knock-in at a unique locus, suggesting that this system could help to expand the feasibility of the zygote-mediated generation of genome-modified animals.

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