Sequence imperfections and base triplet recognition by the Rad51/RecA family of recombinases [Molecular Biophysics]

Homologous recombination plays key roles in double strand break repair, rescue and repair of stalled replication forks, and meiosis. The broadly conserved Rad51/RecA family of recombinases catalyze the DNA strand invasion reaction that takes place during homologous recombination. We have established ssDNA curtain assays for measuring individual base triplet steps during the early stages of strand invasion. Here we examine how base triplet stepping by RecA, Rad51, and Dmc1 is affected by DNA sequence imperfections, such as single and multiple mismatches, abasic sites, and single nucleotide insertions. Our work reveals features of base triplet stepping that are conserved among these three phylogenetic lineages of the Rad51/RecA family, and also reveals lineage-specific behaviors reflecting properties that are unique to each recombinase. These findings suggest that Dmc1 is tolerant of single mismatches, multiple mismatches, and even abasic sites, whereas RecA and Rad51 are not. Interestingly, the presence of single nucleotide insertion abolishes recognition of an adjacent base triplet by all three recombinases. On the basis of these findings, we describe models for how sequence imperfections may affect base triplet recognition by Rad51/RecA family members, and we discuss how these models and our results may relate to the different biological roles of RecA, Rad51, and Dmc1.

from #AlexandrosSfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/2qJAe5D
via IFTTT