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Photoaffinity Labeling of Plant Receptor Kinases.
Methods Mol Biol. 2017;1621:59-68
Authors: Shinohara H, Matsubayashi Y
Abstract
Defining the ligand-binding activity of receptors is important because the binding of ligands is the initial reaction in secreted ligand-dependent cell-to-cell communication. Photoaffinity labeling is one of the most efficient biochemical techniques for detecting direct ligand-receptor interactions. Here, we describe photoaffinity labeling to visualize the direct interaction between peptide ligands and their receptors by using photoactivatable and radioactive peptide ligand derivatives.
PMID: 28567643 [PubMed - indexed for MEDLINE]
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