Publication date: September 2018
Source:Journal of Environmental Radioactivity, Volume 189
Author(s): Reiko Nihei, Mizuki Usami, Taro Taguchi, Seigo Amachi
Previously, we hypothesized that microbial laccase oxidizes iodide (I−) in soils to molecular iodine (I2) or hypoiodous acid (HIO), both of which are easily incorporated into natural soil organic matter, and thus plays a role in iodine sorption on soils. In this study, soil iodide oxidase activity was determined by a colorimetric assay to evaluate if laccase is responsible for iodide oxidation in soils. Three types of Japanese soil showed significant iodide oxidase activities (0.751–2.87 mU g soil−1) at pH 4.0, which decreased with increasing pH, until it was no longer detected at pH 5.5. The activity was inhibited strongly by autoclaving or by the addition of common laccase inhibitors. Similar tendency of inhibition was observed in soil laccase activity, which was determined with 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as a substrate. Significant positive correlations (R2 values of 0.855–0.896) between iodide oxidase activity and laccase activity were observed in two of three soils. Commercially available fungal laccases showed only very low iodide oxidase activities (4.68–18.0 mU mg−1), but enhanced activities of 102–739 mU mg−1 were observed in the presence of redox mediators. Finally, we successfully isolated fungal strains with iodide-oxidizing phenotype in the presence of redox mediators. Polyacrylamide gel electrophoresis of the culture supernatant of Scytalidium sp. strain UMS and subsequent active stain revealed that the fungal laccase actually oxidized iodide in the presence of redox mediators. These results suggest that at least part of iodide in soils is oxidized by fungal laccase through the laccase-mediator system.
from Imaging via alkiviadis.1961 on Inoreader https://ift.tt/2HnfXy0
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Δημοφιλείς αναρτήσεις
-
Publication date: Available online 4 January 2018 Source: European Journal of Radiology Author(s): Peiyao Zhang, Jing Wang, Qin Xu, Zhen...
-
Related Articles Anatomic parameters of the sacral lamina for osteosynthesis in transverse sacral fractures. Surg Radiol Anat....
-
Medicine by Alexandros G. Sfakianakis,Anapafseos 5... Medicine by Alexandros G. Sfakianakis,Anapafseos 5... Heralding change: The evolution ...
-
Background Hyperthyroidism is associated with increased thrombotic risk. As contact system activation through formation of neutrophil extrac...
-
BACKGROUND AND PURPOSE: Dengue is a common arboviral disease, which uncommonly involves the brain. There has been a recent surge in dengu...
-
Abstract Purpose Investigate in patients with metastatic and/or irresectable colorectal cancer treated with systemic treatment with cape...
-
Publication date: January 2017 Source: The Journal of Emergency Medicine, Volume 52, Issue 1 Author(s): Patricia Michelle Troxell Klingen...
-
In rural Kenya, traditional and faith healers provide an alternative pathway to health care, including mental health care. However, not much...
-
2016-12-10T02-17-59Z Source: Indo American Journal of Pharmaceutical Research R. Santosh Kumar*, T. Naga Satya Yagnesh. Solid dispersio...
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου