Sevoflurane induces liver injury by modulating the expression of insulin-like growth factor 1 via miR-214

Abstract

This study aimed to detect the effect of sevoflurane anesthesia on liver injury through modulating IGF-1. The expression of IGF-1 and IGF-1R in liver tissues of sevoflurane-exposed rats was examined by qRT-PCR and western blot. The expression levels of miR-214 in liver cells treated with different concentration of sevoflurane at different time points were detected by qRT-PCR. Enzyme-linked immunosorbent (ELISA) assay was used to analyze serum IGF-1 concentration in cell culture media. After pre-treatment with 100 nM miR-214 inhibitor followed by exposure to sevoflurane, the expression level of miR-214 and IGF-1 protein in liver cells was examined. Hematoxylin-Eosin (HE) staining and TUNEL assay was performed to analyze liver tissue necrosis and apoptosis. The expression levels of apoptosis-related proteins (caspase 3 and Bcl-xL) were examined using western blot. The mRNA and protein expression level of IGF-1 and IGF-1R in rats was significantly down-regulated after 90 min exposure to sevoflurane. QRT-PCR results suggested that exposure to sevoflurane upregulated the expression level of miR-214 and decreased the concentration of IGF-1 in a dose and time dependent manner. Sevoflurane inhibited the expression of IGF-1 through up-regulating miR-214. IGF-1 inhibited the positive effect of sevoflurane on cell necrosis and apoptosis. Sevoflurane could induce liver injury by modulating IGF-1 expression via miR-214. This article is protected by copyright. All rights reserved

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