Increased Visfatin Expression is Associated with Nuclear Factor-kappa β in Obese-Ovalbumin Sensitized Male Wistar Rats Trachea

Objective: To investigate the effects of diet-induced obesity on the expression of nuclear factor-kappa β and visfatin messenger RNA in male Wistar rats’ tracheae after sensitization with ovalbumin (OVA). Materials and Methods: Twenty (20) male Wistar rats were divided into four groups (n=5 for each group), that included a control fed normal diet (ND), and fed normal diet OVA-sensitized (S+ND), high-fat diet (HFD) only, (diet induced obesity) and high-fat diet OVA-sensitized (S+HFD). All animals were fed for 8 weeks with standard pelts or high-fat diet, and then were sensitized and challenged with OVA or saline for another 4 weeks as per the above groups. The rats anesthetized, then the necks were exposed, the tracheae isolated and examined for expression levels of NF-κβ and visfatin mRNA with real time polymerase chain reaction method. Data were compared between different groups using one-way analysis of variance. Results: The expression levels of NF-κβ mRNA in S+HFD: 2.67 was statistically higher than in ND: 0.96 (p=0.001), S+ND: 1.86 (p=0.05), and HFD: 1.26 (p=0.001). Also, visfatin mRNA expression level in S+HFD: 4.21 was higher than ND: 0.92 and S+ND: 1.79 and HFD: 2.20 (p=0.001). Conclusion: In this study, the expression levels of NF-κβ and visfatin were markedly higher in high-fat diet sensitized rat compared with other groups. These findings indicate that alternative signaling pathways might be activated in diet-induced obesity associated with OVA-sensitized animal model and could be responsible for possible altered sensitization phenotype.


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