2016-10-16T02-20-53Z
Source: Indo American Journal of Pharmaceutical Research
Prashant Kale,*, Y. K. Agrawal.
A purpose was to develop and validate a simple, sensitive, high throughput LC-MS/MS method for quantification of mirabegron in human plasma. Mirabegron is a selective agonist for human beta 3-adrenoceptor. The method involves solid-phase 96 well plate extraction using mirabegron-d5 as an internal standard (ISTD). Chromatographic separation was achieved on Unisol C18 3μ 100Ǻ 4.6 X 100mm column with a mobile phase consist of 0.1% ammonia solution (v/v) and methanol (40:60 v/v) at a flow rate of 1.0mL/min and the total run time was 4.0 min. Detection was carried out through positive electro-spray ionization mode by multiplexing of two product ions. Finally the method was developed with the lower limit of quantification as low as 0.1 ng/ml. The method was validated for linearity, precision, accuracy and stability. All the results were within the pre defined acceptance criteria. The method is ready to use in a pharmacokinetic studies.
http://ift.tt/2e85jN4
Κυριακή 16 Οκτωβρίου 2016
A MASS-SPECTROMETRIC 96-WELL PLATE ASSAY FOR THE DETERMINATION OF MIRABEGRON IN HUMAN PLASMA FOR THE APPLICATION IN PHARMACOKINETIC STUDIES.
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Δημοφιλείς αναρτήσεις
-
Publication date: Available online 4 January 2018 Source: European Journal of Radiology Author(s): Peiyao Zhang, Jing Wang, Qin Xu, Zhen...
-
Publication date: March 2017 Source: Free Radical Biology and Medicine, Volume 104 from #AlexandrosSfakianakis via Alexandros G.Sfak...
-
Dtsch med Wochenschr DOI: 10.1055/s-0043-100054 Hintergrund und Fragestellung Ein etablierter Weg, die optimale Behandlung von Tumorpatien...
-
Antibodies, Vol. 9, Pages 21: Construction of Ant... In Vivo and In Vitro Evaluation of Bull Semen Pro... Vertebral artery fenestration mimi...
-
Abstract Purpose F-18 fluorodeoxyglucose (FDG) positron emission tomography (PET) is emerging to be a useful tool in supporting the diag...
-
Does CBD Oil Lower Blood Pressure? This article was originally published at SundayScaries." Madeline Taylor POSTED ON January 13, 20...
-
Publication date: December 2017 Source: Advances in Biological Regulation, Volume 66 Author(s): Lauren Rusnak, Haian Fu The mitogen-activ...
-
Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182, Butyric Acid from Probiotic Staphyloco...
-
Correction to: The IL-23p19/EBI3 heterodimeric cytokine termed IL-39 remains a theoretical cytokine in man The original article can be found...
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου