Serum TCTP is involved in rat liver regeneration after hepatectomy.

Serum TCTP is involved in rat liver regeneration after hepatectomy.

Hepatol Res. 2016 Mar 11;

Authors: Hao S, Qin Y, Yin S, He J, He D, Wang C

Abstract
AIMS: The translationally controlled tumor protein (TCTP) has been reported to promote progression of many physiological processes. However, whether TCTP is involved in liver regeneration has been rarely studied. This study aimed to investigate the potential role of serum TCTP in liver regeneration after 2/3 partial hepatectomy.
METHODS: The synthesis rate and accumulated expression of TCTP was assessed by phosphor imaging and Western blotting, respectively. The mRNA expression of tctp was analyzed by quantitative real-time PCR. The effect of serum TCTP on hepatocyte proliferation was investigated by bromodeoxyuridine (BrdU) incorporation, liver/body weight ratio, albumin concentration and histological examination of liver following treatment of rat with anti-TCTP antibody or prokaryotic TCTP protein before hepatectomy. The MTT assay was used to examine effect of TCTP on hepatocyte proliferation in vitro.
RESULTS: The results showed that the expression of intracellular and serum TCTP protein was significantly increased in rats after 2/3 partial hepatectomy (PHx). In vivo BrdU labeling assay suggested that administration of anti-TCTP antibody before hepatectomy significantly decreased hepatocyte proliferation and liver/body weight ratio. The prokaryotic TCTP had potential promoting effect on hepatocyte proliferation both in vivo and in vitro although administration of prokaryotic TCTP into rats prior to hepatectomy did not increase the proliferation ratio and liver/body weight ratio. Furthermore, anti-TCTP antibody pretreatment decreased the expression of cyclinE, cdk2 and interleukin-6 in rat liver.
CONCLUSIONS: These findings suggest serum TCTP is involved in rat liver regeneration through promoting hepatocyte proliferation. This article is protected by copyright. All rights reserved.

PMID: 26969900 [PubMed - as supplied by publisher]

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