Detection of major histocompatibility complex molecules in processed allogeneic bone blocks for use in alveolar ridge reconstruction

Publication date: Available online 20 March 2018
Source:Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Author(s): Tobias Fretwurst, Lames M. Gad, Thorsten Steinberg, Hagen Schmal, Robert Zeiser, Anna-K. Amler, Katja Nelson, Brigitte Altmann
ObjectivesBecause processed allogenic bone blocks contain remnants of cells and other organic material, the present study examined the putative presence of major histocompatibility complex (MHC) molecules in protein extracts derived from processed allogeneic bone blocks.Study DesignProtein content and the immunogenic potential of 3 different processed allografts (Osteograft, DIZG, Berlin, Germany; Caput femoris, DIZG, Berlin, Germany; Human Spongiosa, Charité Tissue Bank, Berlin, Germany) were assessed by protein extraction and analysis of the presence of MHC class 1 and 2 molecules prior to grafting. MHC concentration was measured by using enzyme-linked immunosorbent assay.ResultsProtein content in the allograft materials varied between 0.87 and 1.61 µg protein/mg. In the allograft Human Spongiosa, no MHC was detected, whereas in the allogeneic bone blocks Osteograft and Caput femoris MHC 1 (0.04–0.037 ng/mg graft material) and in Osteograft MHC class 2 molecules were detectable.ConclusionsThe results of the present study suggest that despite thorough processing, a potential antigenicity of allografts is not eliminated. MHC molecules in allografts may sensitize the immune system.



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