Interaction of proteins with lemon-juice/glutathione-derived carbon nanodot: Interplay of induced-aggregation and co-solubilization.

Interaction of proteins with lemon-juice/glutathione-derived carbon nanodot: Interplay of induced-aggregation and co-solubilization.

Int J Biol Macromol. 2018 Feb 07;:

Authors: Bihani O, Rai T, Panda D

Abstract
Carbon nanodots have generated huge interest in biomedical applications owing to unique emission property and good biocompatibility. In this study, a carbon nanodot is synthesized from a natural resource- lemon juice which is consumed in daily life and glutathione, a biologically active molecule. The synthesized nanodot possesses excitation-independent emission and nano-sheet like like with high graphitic content. Interaction of protein with CND is monitored by intrinsic fluorescence (trp residues), FT-IR and circular dichroism spectroscopy. Interestingly, at a lower concentration, nanodot promotes the protein aggregation. Whereas it solubilizes the protein aggregates at its higher concentration. Both induced-aggregation and co-solubilization are sequence-independent and dictated by nanodot. The study may shed light on the role of glutathione in glutathione-dependent glyoxalase system toward defense against glycation product.

PMID: 29427683 [PubMed - as supplied by publisher]

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