Τρίτη 19 Δεκεμβρίου 2017

Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9.

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Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9.

Stem Cell Res. 2017 Nov 10;26:67-71

Authors: Yuan F, Guo D, Liu Y, Xu Y, Gao G, Wu Y, Yang F, Ke X, Lai K, Wei H, Li YX

Abstract
The ASS1 gene encodes argininosuccinate synthetase-1, a cytosolic enzyme with a critical role in the urea cycle. Mutations are found in all ASS1 exons and cause the autosomal recessive disorder citrullinemia. Using CRISPR/Cas9-editing, we established the WAe001-A-13 cell line, which was heterozygous for an ASS1 mutation, from the human embryonic stem cell line H1. The WAe001-A-13 cell line maintained the pluripotent phenotype, the ability to differentiate into all three germ layers and a normal karyotype.

PMID: 29247816 [PubMed - as supplied by publisher]



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