Integrative genomic analysis of pediatric osteosarcoma

Osteosarcoma (OS) is the most common malignant tumor of bone, affecting predominantly children and young adults. Even though modern treatment regimens including pre- and postoperative multidrug chemotherapy and surgical resection, have additional improved 5 year survival rates to currently 60-70%, no significant improvements have been achieved in the past decades. Furthermore, the systemic chemotherapy applied lacks specificity and can lead to severe adverse effects. New targeted cancer treatment approaches, informed by genomic analysis, aim to exploit molecular properties specific to the neoplastic cells. However, the genomic landscape of OS is complex and is characterized by chromosomal instability, which has historically confounded driver gene discovery. While few and small previous genomic studies exist, a higher sample number and state of the art analysis methods can contribute to a more complete picture of recurring and driving events. To this end, we first characterized the genomes of OS using high-throughput sequencing and single nucleotide polymorphism arrays. We found features of TP53 intron 1 rearrangements suggesting a highly specific mechanism correlated with transcription. Screening of 288 OS and 1'090 other tumor types revealed evidence for TP53 rearrangements in 46 (16%) OS, while none were detected in residual tumors, indicating high specificity to OS. Further, we identified a TP53 intron 1 rearrangement as the causal aberration in a four-generation Li-Fraumeni syndrome family and showed that this rearrangement can at least partially explain the diagnostic gap of formerly classified “TP53 wild-type” Li-Fraumeni syndrome. We then sequenced the exomes of 31 OS and integrated the findings with the corresponding sequencing and single nucleotide polymorphism array data from a replication set of 92 tumors. We identified 14 main driver genes, including some which were formerly unknown in the context of OS. More than 80% of analyzed tumors furthermore exhibited a specific combination of single-base substitutions, LOH, or large-scale genome instability signatures which are characteristic of BRCA1/2-deficient tumors. These BRCA-like traits might be of therapeutic potential since they could render the tumor cells susceptible to PARP inhibitor treatment, which might constitute a novel therapeutic strategy to support current chemotherapy regimens. Last, we screened 337 OS patients for germline alterations and identified bona fide pathogenic mutations in the RET proto-oncogene in 2% of cases. These mutations appear to combine functional kinase activity with dysfunctional ligand binding and expose affected individuals to an increased risk of developing OS when compared to the normal population (odds ratio 9.12). Our findings add OS to the spectrum of RET associated diseases and highlight RET as a potential target for multi-targeted tyrosine kinase inhibitors.

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