Specific rebinding of protein imprinted polyethylene glycol grafted calcium alginate hydrogel with different crosslinking degree

Abstract

Bovine serum albumin imprinted polyethylene glycol 600 (PEG600) grafted Calcium alginate (CaA) hydrogel microspheres were prepared and characterized. The adsorption and recognition properties of PEG600 grafted calcium alginate (CaA-g-PEG600) microspheres were evaluated and the results showed that the crosslinking structure of CaA-g-PEG600 microspheres exerted an obvious effect on the adsorption capacity and imprinting properties for bovine serum albumin. The adsorption isotherms and recognition properties indicated that the imprinted modified microspheres had excellent rebinding affinity toward target proteins and the imprinting efficiency varied according to PEG600 grafting degree. The adsorption capacity and the imprinting factor were 5.5 mg g⁻¹ and 3.6, respectively. Adsorption kinetics of CaA-g-PEG600 microspheres in accordance with the molecular weight between crosslinks (Mc) was investigated and the structural influence on protein selective rebinding was discussed. Furthermore, the binary solution separation performance of CaA-g-PEG600 microspheres with different Mc was investigated by selective binding bovine serum albumin from protein mixture solution.

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