Nanocellulose-collagen-apatite composite associated with osteogenic growth peptide bone regeneration

Publication date: October 2017
Source:International Journal of Biological Macromolecules, Volume 103
Author(s): Sybele Saska, Lucas Novaes Teixeira, Larissa Moreira Spinola de Castro Raucci, Raquel Mantuaneli Scarel-Caminaga, Leonardo Pereira Franchi, Raquel Alves dos Santos, Silvia Helena Santagneli, Marisa Veiga Capela, Paulo Tambasco de Oliveira, Catarina Satie Takahashi, Ana Maria Minarelli Gaspar, Younès Messaddeq, Sidney José Lima Ribeiro, Reinaldo Marchetto
Despite advances in the field of biomaterials for bone repair/regeneration, some challenges for developing an ideal bone substitute need to be overcome. Herein, this study synthesized and evaluated in vitro a nanocomposite based on bacterial cellulose (BC), collagen (COL), apatite (Ap) and osteogenic growth peptide (OGP) or its C-terminal pentapeptide [OGP(10-14)] for bone regeneration purposes. The BC-COL nanocomposites were successfully obtained by carbodiimide-mediated coupling as demonstrated by spectroscopy analysis. SEM, FTIR and ³¹P NMR analyses revealed that in situ synthesis to apatite was an effective route for obtaining of bone-like apatite. The OGP-containing (BC-COL)-Ap stimulated the early development of the osteoblastic phenotype. Additionally, the association among collagen, apatite, and OGP peptides enhanced cell growth compared with OGP-containing BC-Ap. Furthermore, none of the nanocomposites showed cytotoxic, genotoxic or mutagenic effects. These promising results suggest that the (BC-COL)-Ap associated with OGP peptides might be considered a potential candidate for bone tissue engineering applications.



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