Effect of therapeutic concentration of lithium on live HEK293 cells; increase of Na+/K+-ATPase, change of overall protein composition and alteration of surface layer of plasma membrane

Publication date: Available online 13 February 2017
Source:Biochimica et Biophysica Acta (BBA) - General Subjects
Author(s): Miroslava Vosahlikova, Hana Ujcikova, Oleksandr Chernyavskiy, Jana Brejchova, Lenka Roubalova, Martin Alda, Petr Svoboda
BackgroundThe effect of long-term exposure of live cells to lithium cations (Li) was studied in HEK293 cells cultivated in the presence of 1mM LiCl for 7 or 21days. The alteration of Na⁺/K⁺-ATPase level, protein composition and biophysical state of plasma membrane was determined with the aim to characterize the physiological state of Li-treated cells.MethodsNa⁺/K⁺-ATPase level was determined by [³H]ouabain binding and immunoblot assays. Overall protein composition was determined by 2D electrophoresis followed by proteomic analysis by MALDI-TOF MS/MS and LFQ. Li interaction with plasma membrane was characterized by fluorescent probes DPH, TMA-DPH and Laurdan.ResultsNa⁺/K⁺-ATPase was increased in plasma membranes isolated from cells exposed to Li. Identification of Li-altered proteins by 2D electrophoresis, MALDI-TOF MS/MS and LFQ suggests a change of energy metabolism in mitochondria and cytosol and alteration of cell homeostasis of calcium. Measurement of Laurdan generalized polarization indicated a significant alteration of surface layer of isolated plasma membranes prepared from both types of Li-treated cells.ConclusionsProlonged exposure of HEK293 cells to 1mM LiCl results in up-regulation of Na⁺/K⁺-ATPase expression, reorganization of overall cellular metabolism and alteration of the surface layer / polar head-group region of isolated plasma membranes.General significanceOur findings demonstrate adaptation of live HEK293 cell metabolism to prolonged exposure to therapeutic concentration of Li manifested as up-regulation of Na⁺/K⁺-ATPase expression, alteration of protein composition and change of the surface layer of plasma membrane.



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