Publication date: July 2016
Source:International Journal of Biological Macromolecules, Volume 88
Author(s): Loredana Dumitrașcu, Nicoleta Stănciuc, Iuliana Aprodu
Thermal dependent conformational changes of xanthine oxidase (XOD) were studied using sensitive and non-destructive methods like fluorescence spectroscopy and molecular modeling in the temperature range of 25–85°C. Intrinsic fluorescence studies showed that the microenvironment of tryptophan and tyrosine residues becomes more exposed to solvent as the temperature increased up to 85°C, whereas in case of flavin cofactor is rather conserved. At higher temperatures, the flavin adenine dinucleotide is displaced from the core of the protein, but is not fully released as shown by the Stern Volmer quenching constant and accessible fraction of the cofactor. Anyway, no significant changes in the structure of XOD monomer were identified after running molecular dynamics simulations at temperatures 25°C, 65°C and 85°C. Therefore, we can conclude that the most important changes in the protein structure at thermal treatment mainly consist on molecular aggregation and dissociation events.
from #AlexandrosSfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/1NdQ78a
via IFTTT
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Δημοφιλείς αναρτήσεις
-
Publication date: Available online 4 January 2018 Source: European Journal of Radiology Author(s): Peiyao Zhang, Jing Wang, Qin Xu, Zhen...
-
Pycnodysostosis is a rare autosomal, recessive, skeletal dysplasia caused by a mutation in the cathepsin k gene. Pycnodysostosis is characte...
-
2016-12-10T02-17-59Z Source: Indo American Journal of Pharmaceutical Research Radha S & Sreelatha .S*. Research has demonstrated th...
-
ACS Nano DOI: 10.1021/acsnano.7b01783 from #AlexandrosSfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/2trY7nv via...
-
2016-10-22T03-07-52Z Source: International Journal of Community Medicine and Public Health Ketan R. Dagdiya, Sushma S. Thakre, Subhash B....
-
Need Industrial relations advice? Unfair dismissals laws? Discrimination in the workplace? Sexual harassment? We can help. AWNA is Australia...
-
by Javier U. Chicote, Rob DeSalle, José Segarra, Tung-Tien Sun, Antonio García-España from #AlexandrosSfakianakis via Alexandros G.Sfakia...
-
Vol.28 No.1 from #AlexandrosSfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/1QLVDop via IFTTT
-
Participant Experiences of Attending a Community CBT Workshop for Insomnia: A Qualitative Six-Year Follow-Up. Behav Sleep Med. 2017 Ap...
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου