The Prozone Effect Accounts for the Paradoxical Function of the Cdk-Binding Protein Suc1/Cks

Publication date: Available online 4 February 2016
Source:Cell Reports
Author(s): Sang Hoon Ha, Sun Young Kim, James E. Ferrell
Previous work has shown that Suc1/Cks proteins can promote the hyperphosphorylation of primed Cdk1 substrates through the formation of ternary Cdk1-Cks-phosphosubstrate complexes. This raises the possibility that Cks proteins might be able to both facilitate and interfere with hyperphosphorylation through a mechanism analogous to the prozone effect in antigen-antibody interactions, with substoichiometric Cks promoting the formation of Cdk1-Cks-phosphosubstrate complexes and suprastoichiometric Cks instead promoting the formation of Cdk1-Cks and Cks-phosphosubstrate complexes. We tested this hypothesis through a combination of theory, proof-of-principle experiments with oligonucleotide annealing, and experiments on the interaction of Xenopus cyclin B1-Cdk1-Cks2 with Wee1A in vitro and in Xenopus extracts. Our findings help explain why both Cks under-expression and overexpression interfere with cell-cycle progression and provide insight into the regulation of the Cdk1 system.

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Teaser

Ha et al. find that Cks2 can both promote and repress Cdk1 phosphorylation of Wee1A in a biphasic manner through its bivalent adaptor-like function. This phenomenon is related to the prozone effect in antigen-antibody interaction and to squelching in transcription.


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