Partially reduced graphene oxide as highly efficient DNA nanoprobe.

Partially reduced graphene oxide as highly efficient DNA nanoprobe.

Biosens Bioelectron. 2016 Jan 21;80:140-145

Authors: Wang YH, Deng HH, Liu YH, Shi XQ, Liu AL, Peng HP, Hong GL, Chen W

Abstract
This work investigates the effect of reduction degree on graphene oxide (GO)-DNA interaction and the fluorescence quenching mechanism. Partial reduced graphene oxide (pRGO), which maintains well water-dispersibility, is synthesized using a mild reduction method by incubating GO suspension under alkaline condition at room temperature. The fluorescence quenching enhances with the restoration degree of sp(2) carbon bonds and follows the static quenching mechanism. The binding constant values imply that pRGO has much stronger affinity with ssDNA than GO. Utilizing this highly efficient nanoprobe, a universal sensing strategy is proposed for homogeneous detection of DNA. Compared with the reported GO-based DNA, this present strategy has obvious advantages such as requirement of low nanoprobe dosage, significantly reduced background, fast fluorescence quenching, and improved sensitivity. Even without any amplification process, the limit of detection can reach as low as 50pM.

PMID: 26826548 [PubMed - as supplied by publisher]

from #AlexandrosSfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/1Q3oWgY
via IFTTT