ISOLATION AND CHARACTERIZATION OF AMYLASE PRODUCING BACTERIA FROM ORANGE AND POMEGRANATE PEEL

2016-12-10T02-17-59Z
Source: Indo American Journal of Pharmaceutical Research
G.Prameela, K. Priya Dharshini, M.Chidanandappa.K.Kamala*.
The enzymes from microbial sources are more stable and obtained cheaply. Amylases are among the most important enzymes and are of great significance in present day industry. Starch degrading bacteria are most important for industries such as food, fermentation, textile and paper. Thus isolating and manipulating pure culture from various soils has manifold importance for various biotechnology industries. Amylase production from bacteria is economical as the enzyme production rate is higher in bacteria as compared to other microorganism. The aim of the current study was to isolate amylase producing bacteria from the soil samples collected. The isolation was done by serial dilution and plating method. Characteristic feature of the strains indicates them as Bacillus sps. Bacterial isolates were screened for amylolytic activity by starch agar plate method. The optimum ph for production was 7, whereas maximum growth was observed at 1gm Orange Peel and pomegranate Powder. The concentration of enzyme increases with increase in substrate concentration. The pH range was found to be 7 and incubation time 48hrs with 1ml as inoculum for optimum growth.


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