Markers to sensibility and relapse on IMR-32 neuroblastoma cell line cultured in monolayer (2D) and neurosphere (3D) models cisplatin-treated

xlomafota13 shared this article with you from Inoreader Markers to sensibility and relapse on IMR-32 neuroblastoma cell line cultured in monolayer (2D) and neurosphere (3D) models cisplatin-treated Via histochem Acta Histochem. 2022 Jan 13;124(2):151849. doi: 10.1016/j.acthis.2022.151849. Online ahead of print. ABSTRACT The complexity of different components of tumor stroma poses huge challenges for therapies targeting the neuroblastoma (NB) microenvironment. The present study aimed to evaluate platinum-based response in IMR-32 neuroblastoma cell line cultured in monolayer (2D) and neurosphere (3D) models. For this, we evaluated mRNA expression of heat shock proteins HSPA1A, HSPB1, TRAP1, HSPA1AL, HSPD1, and DNA damage repair gene ERCC1. After treatment, residual cells were grafted on CAM (chicken chorioallantoic membrane) to evaluate the growth capability and histological paraffin sections were made to assess Ki-67 and HER-2 proteins by immunofluorescence. Our results showed that cisplatin induces mRNA downregulation of Heat Shock Proteins and ERCC1 in IMR-32 cells cultured in 2D or 3D models. In addition, the cisplatin-treatment approach increased HER-2 expression in residual IMR-32 cells grafted on the CAM. Therefore, these insights provide many advances in neuroendocrine tumor biology and knowledge about cisplatin-response in neuroblastoma. PMID:35033934 | DOI:10.1016/j.acthis.2022.151849 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Respiratory problems and associated factors following endoscopic balloon dilatation procedure in children with acquired subglottic stenosıs

xlomafota13 shared this article with you from Inoreader Respiratory problems and associated factors following endoscopic balloon dilatation procedure in children with acquired subglottic stenosıs Via http://link.springer.com/journal/405,European archives of oto-rhino-laryngology,The European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino Eur Arch Otorhinolaryngol. 2022 Jan 17. doi: 10.1007/s00405-021-07208-3. Online ahead of print. ABSTRACT OBJECTIVES: Endoscopic balloon dilatation (EBD) offers a safe and non-invasive surgical option for the treatment of subglottic stenosis. Patient selection is important to achieve good results and to detect which patients are more prone to the development of complications. The aim of this study was to determine predictors of postoperative problems and early complications in primary EBD surgeries. METHODS: A retrospective analysis was made of patients with acquired subglottic stenosis who were operated on with the EBD technique between January 2010 and December 2019 in the Otolaryngology-Head and Neck Surgery Department of Baskent University Hospital. Demographic data including the age and sex of the patients were collected together with etiology, presence of chromosomal or craniofacial anomaly (C/CA), duration of prolonged intu bation (DPI), and extubation dilatation timeframe (EDT). Intra and postoperative follow-up data were recorded of the need for intubation or tracheotomy, development of desaturation, and grade and type of stenosis. RESULTS: The male to female ratio was 2:1. The patients comprised 42 males and 22 females with a mean age of 296.52 ± 551.93 days. The cause of prolonged intubation was surgery for congenital heart disease in 50 (78.1%) patients and prematurity in 14 (21.9%). The type of lesion was acute granulation in 44 (72.1%) and chronic granulation in 17 (27.9%) patients. C/CA was determined in 13 patients, the mean grade of stenosis was 76.33 ± 15.21%, mean DPI was 25.25 ± 35.49 days, and mean EDT was calculated as 78.23 ± 373.82 days. Desaturation following endoscopic balloon dilatation developed in 26 (40.6%), orotracheal intubation was required in 10 (15.6%), tracheotomy in 10 (15.6%), and cardiopulmonary arrest occurred in 4 (6.25%). Prematurity, a longer duration of pr eoperative intubation, longer time from extubation to dilatation, older age, and higher grade of stenosis were determined as factors associated with postoperative early respiratory complications. CONCLUSION: EBD indication should be carefully considered in children with acquired subglottic stenosis. To achieve better results and minimise complications, EBD should be performed without delay. PMID:35037169 | DOI:10.1007/s00405-021-07208-3 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Immunotherapy of head and neck cancer : Highlights of the ASCO and ESMO annual meetings 2021

xlomafota13 shared this article with you from Inoreader Immunotherapy of head and neck cancer : Highlights of the ASCO and ESMO annual meetings 2021 Via hno HNO. 2022 Jan 17. doi: 10.1007/s00106-021-01142-w. Online ahead of print. ABSTRACT BACKGROUND: This year's American Society of Clinical Oncology (ASCO) meeting included interesting data on first-line therapy of nasopharyngeal carcinomas with PD‑1 inhibitors and on checkpoint inhibition in various clinical constellations. At the European Society of Medical Oncology (ESMO) meeting, the results of the CheckMate-651 study were presented. MATERIALS AND METHODS: All abstra cts and presentations from the ASCO and ESMO meetings 2021 on immunotherapy in head and neck cancer (HNSCC) were evaluated for their relevance. The most interesting studies are elaborated upon herein. RESULTS: Studies on locally advanced HNSCC showed an improved response after neoadjuvant pembrolizumab administration. A second cycle did not improve the response rate, but the proportion of patients with a good response was almost doubled. The CheckRad CD8 study showed an improvement in progression-free survival by induction chemoimmunotherapy with tremelimumab and durvalumab followed by stratification according to the CD8 immune cell infiltrate. Two studies were presented on first-line treatment of recurrent/metastatic nasopharyngeal carcinomas. Chemoimmunotherapy showed a higher response rate and prolonged progression-free survival with a similar adverse event profile. In recurrent/metastatic HNSCC, the CheckMate 651 study showed an increased duration of response with nivoluma b and ipilimumab and higher response rates than pembrolizumab alone. The primary endpoints for overall survival were not achieved. CONCLUSION: PD‑1 inhibition has great potential to change the therapeutic landscape for nasopharyngeal carcinomas in the future. In HNSCC, CD8 tumor infiltrate presents a promising predictive marker for selecting patients who can benefit from radioimmunotherapy. The combination of nivolumab and ipilimumab did not improve overall survival in palliative first-line therapy; thus, no change in the current standard is expected. PMID:35037989 | DOI:10.1007/s00106-021-01142-w View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

A Rare Cause of Secondary Otalgia

xlomafota13 shared this article with you from Inoreader Message: PrivacyPrivacy Skip to main content Intended for healthcare professionals SAGE Journals Search search-icon Browse Resources Access Options: You are not signed in to your personal profile Sign In You are not signed in via an institution Institution You are not authenticated via a society Society Cart Ear, Nose & Throat Journal 1.697 Impact Factor 5-Year Impact Factor 1.745 Journal Indexing & Metrics » Journal Home Browse Journal Journal Info Stay Connected Submit Paper Search Article Menu Download PDF [PDF] Open EPUB Full Article Content List Significance Statement ORCID iDs References Figures & Tables Article Metrics Cite Share Request Permissions Related Articles A Rare Cause of Secondary Otalgia Show all authors Evropi Forozidou, MD, Nikolaos Tsetsos, MD, MSc, Paraskevi Karamitsou, MD, MSc, ... First Published January 17, 2022 Research Article https://doi.org/10.1177/01455613221075226 Article information Open AccessCreative Commons Attribution, Non Commercial 4.0 License Significance Statement Secondary otalgia is defined as pain felt in the ear although originating from a non-otologic source. The complex innervation of ear structures makes the identification of the responsible region a challenging procedure. The 2 most common causes of secondary otalgia are the temporomandibular joint dysfunction and dental infections. We present a rare case of secondary otalgia caused by a foreign body hidden deeply in the lateral surface of the tongue. A 61-year-old male ironworker presented to our emergency Ear, Nose and Throat department complaining about left otalgia accompanied by difficulty in swallowing. Symptoms had started 1 week before in his work environment. The patient was prescribed a 5-day course of antibiotics with ciprofloxacin ear drops combined with painkillers by his family doctor without, however, any signs of improvement. His past medical history was otherwise normal. A thorough clinical examination combined with otomicroscopy was unremarkable for any ear pathology. Fiberoptic nasolaryngoscopy and laboratory tests were also normal. Inspection of the oral cavity showed no signs of inflammation; however, a tender area on the left lateral surface of the tongue was noted. After careful observation, a tiny hole was recognized in the same area (Figure 1). An exploration of the area under local anesthesia was conducted and a metallic iron bar of approximately 1.5 cm in length was removed (Figure 2). Symptoms were completely subsided and the patient remained pain free at 1-week follow-up. figure Figure 1. Oral cavity inspection. Recognition of the painful area on the left lateral surface of the tongue. figure Figure 2. The extracted foreign body. A metallic iron bar. Otalgia is a rather common symptom seen in the primary care setting with many diverse causes. Primary otalgia is related to clinical entities affecting the outer, middle, and inner ear.1 Inflections such as acute or chronic media otitis, external otitis, folliculitis, mastoiditis, and myringitis constitute the most common etiologic factors. Cerumen obstruction, ear neoplasms, and trauma may also be responsible for primary otalgia. The origin of primary otalgia is almost always easy to be established with otomicroscopy or radiographic imaging.2 On the other hand, when the cause of pain cannot be localized to the affected ear, it is referred to as secondary otalgia. There is a considerable overlap between the innervation of the ear and the related areas in the head and neck. Innervation of the ear structures comprises multiple lower cranial, upper cervical, and peripheral nerves. They innervate the spine, skull base, salivary glands, pharynx, larynx, oral cavity, orbits, face, paranasal sinuses, and deep neck spaces. The most common causes of secondary otalgia are temporomandibular joint syndrome and dental infections. Additionally, other potential causes of otalgia are Bell's palsy, salivary gland disorders, pharyngitis, tonsillitis, oral disorders, and cervical osteoarthritis.2,3 Clinicians should be aware that otalgia could be the primary symptom of a head and neck malignancy. Therefore, a thorough clinical examination of the whole head and neck area is imperative to exclude neoplasms.3,4 Inflammation, trauma, and neoplasms of the tongue often cause secondary otalgia via the trigeminal (CN V) and the glossopharyngeal nerve (CN IX). The third branch of the trigeminal, the mandibular nerve (V3), is a mixed nerve. The auriculotemporal nerve is a branch of the V3 that provides sensation to the anterosuperior pinna, anterior external auditory canal, and the anterior lateral aspect of the tympanic membrane. Other branches include the lingual, buccal, and inferior alveolar nerves that provide sensory innervation to the oral cavity, the floor of the mouth, and the anterior two-thirds of the tongue.5 The glossopharyngeal nerve (CN IX) directly innervates the inner surface of the tympanic membrane as well as the middle ear cavity through sensory fibers of the tympanic nerve (Jacobson nerve). It also provides mixed innervation to the posterior third of the tongue.6 Secondary otalgia may be caused from anywhere along the course of this nerve. In cases that thorough clinical investigation fails to establish the source of otalgia, a computed tomography or magnetic resonance imaging studies should be considered to define the diagnosis.5 Declaration of Conflicting Interests The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. Funding The author(s) received no financial support for the research, authorship, and/or publication of this article. ORCID iDs Nikolaos Tsetsos https://orcid.org/0000-0003-1884-6824 Konstantinos Garefis https://orcid.org/0000-0003-3905-5650 Alexandros Poutoglidis https://orcid.org/0000-0002-4591-8347 References 1. Neilan, RE, Roland, PS. Otalgia. Med Clin North Am. 2010;94:96171. Google Scholar | Crossref 2. Norris, CD, Koontz, NA. Secondary Otalgia: Referred Pain Pathways and Pathologies. AJNR Am J Neuroradiol. 2020;41(12):2188-2198. Google Scholar | Crossref | Medline 3. Earwood, JS, Rogers, TS, Rathjen, NA. Ear pain: diagnosing common and uncommon causes. Am Fam Physician. 2018;97(1):20-27. Google Scholar | Medline 4. Charlett, SD, Coatesworth, AP. Referred otalgia: a structured approach to diagnosis and treatment. Am J Med Sci Med. 2017;5(3):56-61. Google Scholar 5. Scarbrough, TJ, Day, TA, Williams, TE, et al. Referred otalgia in head and neck cancer: a unifying schema. Am J Clin Oncol. 2003;26:e157-e162. Google Scholar | Crossref | Medline 6. Naraev, BG, Linthicum, FH. Traumatic neuroma of the tympanic (Jacobson's) nerve as a possible cause of otalgia. Otolaryngol Head Neck Surg. 2008;138:735-737. Google Scholar | SAGE Journals View Abstract SAGE Recommends Also from SAGE Publishing CQ LibraryAmerican political resourcesopens in new tab Data PlanetA universe of dataopens in new tab Lean LibraryIncrease the visibility of your libraryopens in new tab SAGE Business CasesReal-world cases at your fingertipsopens in new tab SAGE CampusOnline skills and methods coursesopens in new tab SAGE KnowledgeThe ultimate social science libraryopens in new tab SAGE Research MethodsThe ultimate methods libraryopens in new tab SAGE VideoStreaming video collectionsopens in new tab SAGE Journals About Privacy Policy Terms of Use Contact Us Help Accessibility Browse Health Sciences Life Sciences Materials Science & Engineering Social Sciences & Humanities Journals A-Z Discipline Hubs Resources Authors Editors Reviewers Librarians Researchers Societies Opportunities Advertising Reprints Content Sponsorships Permissions Microsites Ear, Nose & Throat Journal ISSN: 0145-5613 Online ISSN: 1942-7522 Copyright © 2022 by SAGE Publications A Rare Cause of Secondary Otalgia Via ear nose throat Ear Nose Throat J. 2022 Jan 17:1455613221075226. doi: 10.1177/01455613221075226. Online ahead of print. NO ABSTRACT PMID:35037504 | DOI:10.1177/01455613221075226 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Benefit on daily listening with technological advancements: comparison of basic and premium category hearing aids

xlomafota13 shared this article with you from Inoreader Benefit on daily listening with technological advancements: comparison of basic and premium category hearing aids Via http://link.springer.com/journal/405,European archives of oto-rhino-laryngology,The European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino Eur Arch Otorhinolaryngol. 2022 Jan 17. doi: 10.1007/s00405-021-07240-3. Online ahead of print. ABSTRACT PURPOSE: The aim of the study was to compare the user-rated benefit of two categories of hearing aids, mainly the basic and premium categories of hearing aids. METHODS: A questionnaire was administered on 102 hearing aids users (47 basic and 55 premium category users) with severity of hearing loss ranging from mild to moderately severe sensorineural hearing loss. The questionnaire administered was divided into mainly seven subscales which included speech intelligibility in quiet and in noise, ease of communication, the efficiency of noise reduction, localization, quality of music perception and cost effectiveness. The effect of duration of daily usage of hearing aids on performance among these different subscales was also assessed. RESULTS: Ease of communication was rated better by premium hearing aid users, whereas the cost effectiveness was rated to be better by basic users. There was no significant difference observed between performances of basic versus premium category of hearing aids in other listening domains assessed. There was no significant difference in any of the listening domains with daily usage duration for both categories of hearing aid users. CONCLUSION: The users of premium category devices revealed better ease of communication in daily environments, whereas performance of these devices on other listening domains remains questionable. Cost effectiveness was reported to be better by the users of basic hearing aids. A prospective and controlled paired series comparison of hearing aid performance needs to be performed to confirm these findings. PMID:35038028 | DOI:10.1007/s00405-021-07240-3 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.