Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN

alexandrossfakianakis shared this article with you from Inoreader Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN via International Journal of Oral Science - Issue International Journal of Oral Science, Published online: 19 July 2022; doi:10.1038/s41368-022-00192-2 Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN View on Web

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Comparative study of DFAT cell and ADSC sheets for periodontal tissue regeneration:in vivo and in vitro evidence

alexandrossfakianakis shared this article with you from Inoreader Comparative study of DFAT cell and ADSC sheets for periodontal tissue regeneration:in vivo and in vitro evidence via Journal of Clinical Periodontology Abstract Aim To compare the efficacy of adipocyte-derived dedifferentiated fat (DFAT) cell and adipose-derived stromal cell (ADSC) sheets for regenerative treatment of intrabony periodontal defects. Material and Methods DFAT cells were obtained using the ceiling culture method and were compared with ADSCs using Cell Counting Kit-8 (CCK8), colony formation assay, surface antigen identification, and multilineage differentiation assays. DFAT and ADSC sheets were prepared in cell sheet culture medium. The biological characteristics of DFAT cell and ADSC sheets were compared using haematoxylin and eosin staining, quantitative reverse transcription PCR, and immunofluorescence staining. Micro-computed tomography and histological staining were used to compare the effects of the two cell sheets on the repair of periodontal intrabony defects in rats. Results DFAT cells and ADSCs demonstrated mesenchymal stem cell characteristics. Both cell type were CD29-, CD90-, and CD146-positive and CD31-, CD34-, and CD45-negative. DFAT cells and ADSCs exhibited similar osteogenic and adipogenic differentiation capabilities, and colony-formation ability. DFAT cells displayed stronger proliferation capabilities compared to ADSCs. Compared with the ADSC sheets, DFAT cell sheets exhibited a higher expression of periodontal-related genes and proteins and greater ability to regenerate periodontal tissue. Conclusion Our findings suggest that DFAT cell sheets are an ideal seed cell source and form of cell delivery for periodontal intrabony defects. View on Web

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The accuracies of three intraoral scanners with regards to shade determination: An in vitro study

alexandrossfakianakis shared this article with you from Inoreader The accuracies of three intraoral scanners with regards to shade determination: An in vitro study via Journal of Prosthodontics Abstract Purpose : To compare the accuracy of three intraoral scanners for shade determination function in vitro, and to preliminarily investigate the shade-matching characteristics of the three intraoral scanners. Materials & Methods The shade of the middle third region of each shade tab on the Vita Classical A1-D4 shade guide (VC) was measured by a spectrophotometer (Vita Easyshade V, VE) and three intraoral scanners, including CEREC Omnicam (OM), 3Shape TRIOS 3 (T3), and TRIOS 4 (T4). A conversion table between VC values and CIELAB values was established from the database of VE to analyze the trueness. The reproducibility of the instruments was then compared by repeating the measurements five times. Results : The mean color difference for each instrument was highest in the OM, followed by the T4, and lowest in the T3 and VE, repectively. The L* and a* value for OM, and the b* value for T4, were significantly different from those for VE (p <0.05). The reproducibility of the instrument was highest in the VE (Fleiss' kappa: 0.95), followed by the T3 (Fleiss' kappa: 0.89), T4 (Fleiss' kappa: 0.87), and OM (Fleiss' kappa: 0.78). Conclusions : Of the three intraoral scanners, the trueness was best on the T3. The reproducibility of all the instruments was excellent. This article is protected by copyright. All rights reserved View on Web

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Osteocutaneous Radial Forearm Free Flap Fixed to a Prior Osteocutaneous Free Flap: Two Case Reports

alexandrossfakianakis shared this article with you from Inoreader Osteocutaneous Radial Forearm Free Flap Fixed to a Prior Osteocutaneous Free Flap: Two Case Reports via The Laryngoscope Reconstruction of mandibular defects is best accomplished by composite bony tissue. When the fibula is not available other sources must be used. Occasionaly tumor recurence will neccesitate a further resection and bony reconstruction. We report two cases in which osteocutaneous radial forearm free tissue transfer was used for secondary reconstructio after prior bony free flap reconstruction. Laryngoscope, 2022 View on Web

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The malignant property of circHIPK2 for angiogenesis and chemoresistance in non-small cell lung cancer

alexandrossfakianakis shared this article with you from Inoreader The malignant property of circHIPK2 for angiogenesis and chemoresistance in non-small cell lung cancer via ScienceDirect Publication: Experimental Cell Research Publication date: Available online 19 July 2022 Source: Experimental Cell Research Author(s): Mingming Ren, Xiang Song, Jieting Niu, Guojie Tang, Zhen Sun, Yanguang Li, Fanyi Kong View on Web

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Orthopedic Surgery Causes Gut Microbiome Dysbiosis and Intestinal Barrier Dysfunction in Prodromal Alzheimer Disease Patients: A Prospective Observational Cohort Study

alexandrossfakianakis shared this article with you from Inoreader Orthopedic Surgery Causes Gut Microbiome Dysbiosis and Intestinal Barrier Dysfunction in Prodromal Alzheimer Disease Patients: A Prospective Observational Cohort Study via Annals of Surgery - Most Popular Articles Objective: To investigate gut microbiota and intestinal barrier function changes after orthopedic surgery in elderly patients with either normal cognition (NC) or a prodromal Alzheimer disease phenotype (pAD) comprising either subjective cognitive decline (SCD) or amnestic mild cognitive impairment (aMCI). Background: Homeostatic disturbances induced by surgical trauma and/or stress can potentially alter the gut microbiota and intestinal barrier function in elderly patients before and after orthopedic surgery. Methods: In this prospective cohort study, 135 patients were subject to preoperative neuropsychological assessment and then classified into: NC (n=40), SCD (n=58), or aMCI (n=37). Their gut microbiota, bacterial endotoxin (lipopolysaccharide), tight junction (TJ) protein, and inflammatory cytokines in blood were measured before surgery and on postsurgical day 1, 3, and 7 (or before discharge). Results: The short-chain fatty acid (SCFA)-producing bacteria were lower while the gram-negative bacteria, lipopolysaccharide and TJ were higher preoperatively in both the SCD and aMCI (pAD) groups compared with the NC group. After surgery, a decrease in SCFA-producing bacteria, and an increase in both gram-negative bacteria and plasma claudin were significant in the pAD groups relative to the NC group. SCFA-producing bacteria were negatively correlated with TJ and cytokines in pAD patients on postsurgical day 7. Furthermore, surgery-induced perioperative metabolic stress and inflammatory responses were associated with gut microbiota alterations. Conclusions: Surgery exacerbates both preexisting microbiota dysbiosis and intestinal barrier dysfunction in pAD patients, all of which may be associated with systemic inflammation and, in turn, may lead to further cognitive deterioration. View on Web

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