Evaluation of wound healing activity of cow urine ark in diabetic Wistar albino rats

2016-09-25T15-23-50Z
Source: Journal of Intercultural Ethnopharmacology
Hiren N Hirapara, Vishal M Ghori, Ashish P Anovadiya, Chandrabhanu R Tripathi.
 In present study, effects of cow urine ark on diabetic wound healing was studied.  Wistar Albino rats were used for this study due to their susceptibility for diabetes with streptozotocin and they are established model for diabetic wound healing studies.  Excision, incision and dead space wound model were selected for evaluation of wound healing activities.  Streptozotocin (50 mg / kg) was given i.p. to produce diabetes. Random blood sugar was measured using glucometer from drop of blood from tail vein.  Cow urine ark (5.5 ml / kg) was given orally in the morning.  Excision wounds were traced on transparent sheet as well as photographed and analyzed for wound contraction and re-epithelization with help of UTHSCA image analyzer software. Incision wounds were analyzed for wound breaking strength with help of continuous water flow technique of Lee. Dead space wounds were analyzed for dry granuloma weight, hydroxyproline content and histopathological changes.  Cow urine ark significantly improved the collagen content and granulation tissue formation in diabetic wounds which can be useful in accelerating wound healing in diabetic patients. Clinical implementation would require clinical trials.


http://ift.tt/2db2hDE

Effects of herbal medicine on human uterine tumor-bearing nude mice

2016-09-25T14-53-40Z
Source: Journal of Intercultural Ethnopharmacology
Mi Hyang Ohh, Seong Jin Kim, Jong Kwon Han, Sok Cheon Pak, Kew-mahn Chee.
Aim: Uterine leiomyomas are the most common benign uterine neoplasms associated with significant morbidity. Herbal formulas capable of restoring yin-yang balance by dispersing blood stasis may be useful for managing fibroid symptoms. Materials and Methods: In this study, the antitumor properties of three herbs viz., Trogopterus xanthipes Milen-Edwards, Paeonia lactiflora Pallas, and Ulmus davidiana Planch, were evaluated in nude mice injected intravenously with human malignant myomas. Tumour fragments were xenografted subcutaneously through a flank incision in female mice. The mice entered the study for 8 weeks when their tumors reached the threshold volume (260 mm3). The mice were randomly allocated to receive subcutaneous injections of normal saline (group 1; negative control), P. lactiflora Pallas (group 2), U. davidiana Planch (group 3), T. xanthipes Milen-Edwards (group 4), and intravenous injections of paclitaxel (group 5; positive control). The weight and tumor volume were measured, followed by histopathology. Results: A few cases of abdominal distention and death were observed in the negative control group. Furthermore, a considerable enlargement of the liver and spleen was observed in the negative control group at autopsy with a gradual increase in body weight during the experiment. The mean tumor volume which increased in negative control mice reduced in mice treated with herbal remedies or paclitaxel from day 14 onwards (p

http://ift.tt/2cwOonl

Antidiabetic and antioxidant effects of Croton lobatus l. in alloxan-induced diabetic rats

2016-09-25T14-22-59Z
Source: Journal of Intercultural Ethnopharmacology
Taiye Remi Fasola, Blessing Ukwenya, Ademola Adetkunbo Oyagbemi, Temidayo Olutayo Omobowale, Temitayo Olabisi Ajibade.
Background: Croton lobatus contains high amount of antioxidant phytochemicals that probably account for its wide use as food and medicine in the traditional communities of West Africa. Methods: The study evaluated the modulatory role of methanol extract of Croton lobatus leaf on alloxan-induced diabetes and associated cardiovascular complications in male rats randomly selected and assigned to one of six groups (A to F) of eight animals each: A (distilled water); B (corn oil); C (Alloxan); D (Alloxan + 100 mg kg-1 Croton lobatus); E: (Alloxan + 200 mg kg-1 C. lobatus); and F (Alloxan + 100 mg kg-1 glibenclamide). The extract was administered orally for 14 days. Results: Acute toxicity studies revealed no mortality of rats at with the administration of different doses of extract up to the 5,000 mg kg-1 dose. Diabetic rats treated with 100 and 200 mg kg-1 C. lobatus leaf extract had significant (p

http://ift.tt/2d1MSZl