Purpose. To ex vivo expand oral mucosal epithelium cells (OMECs) on acellular porcine corneal stroma (APCS) without using feeder cells and serum and to compare the morphologic and phenotypic characteristics of cultured oral cells on APCS to those of cells on deluded human amniotic membrane (HAM). Methods. SD rat oral mucosal biopsies were cultured on APCS and HAM. Reverse-transcription polymerase chain reaction (RT-PCR) and immunohistochemistry were used to analyze the characterization of stem cells and epithelial differentiation of the outgrowth products. Results. Stratified and optimal transplantable OMECs were obtained after being cultured three to four weeks. Both RT-PCR and immunohistochemistry showed that cultured OMECs expressed markers of epithelial differentiation cytokeratin K3 and epithelial stem cell markers of p63 and ABCG2. Conclusions. OMECs can be successfully cultured on APCS without using xenobiotic feeder cells and serum. Characterization showed that these sheets retain the morphologic and phenotypic characteristics of OMECs within differentiated cells and stem cells. The optimal transplantable sheets can prove to be particularly beneficial to both bilateral limbal stem cell deficiency and deep corneal lesions.
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Abstract Purpose Overcoming the flaws of current data management conditions in head and neck oncology could enable integrated informatio...
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ACS Nano DOI: 10.1021/acsnano.6b04244 from #AlexandrosSfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/2aOZU8w via...
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JOURNAL OF JAPAN SOCIETY FOR SAFETY ENGINEERING_1998_5 from #AlexandrosSfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt...
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Vol.83 No.3 from #AlexandrosSfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/1TkQfWM via IFTTT
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Small size of metastatic lymph nodes with extracapsular spread greatly impacts treatment outcomes in oral squamous cell carcinoma patie...
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Guidelines for inpatient admission after pediatric tonsillectomy have been proposed to improve the safety of this procedure. This study exam...
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Madhavi Bhargava Journal of Family and Community Medicine 2017 24(2):131-131 from #AlexandrosSfakianakis via Alexandros G.Sfakianakis ...
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Publication date: Available online 14 May 2017 Source: Journal of Oral Biosciences Author(s): Hiromi Kimura-Suda, Teppi Ito BackgroundBo...
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