Increased Expression of Cullin 3 in Nasopharyngeal Carcinoma and Knockdown Inhibits Proliferation and Invasion.

Increased Expression of Cullin 3 in Nasopharyngeal Carcinoma and Knockdown Inhibits Proliferation and Invasion.

Oncol Res. 2017 Apr 18;:

Authors: Zeng R, Tan G, Li W, Ma Y

Abstract
This study aimed to investigate the clinical significance of Cullin 3 expression in nasopharyngeal carcinoma (NPC), aswell as explore the regulatory mechanism of Cullin 3 underlying the growth and metastasis of NPC cells. Our findings showed that the expression levels of Cullin 3 expression were significantly increased in both NPC tissues and cell lines. A strong positive correlation was found between the Cullin 3 expression and Ki67-based proliferation index in NPC tissues. Moreover, Cullin 3 overexpression was correlated with the local relapse and distant metastasis in NPC patients. In vitro experiments showed that knockdown of Cullin 3 caused a significant reduction in the proliferation of NPC cells probably through inducing a cell cycle arrest. Besides, downregulation of Cullin 3 also inhibited the colony formation, migratory and invasive capacities of NPC cells. In addition, the expression levels of PCNA and epithelial to mesenchymal transition (EMT)-related proteins were also meditated by Cullin 3 in NPC cells. Based on these findings above, we demonstrate that Cullin 3 plays a promoting role in the malignant progression of NPC, and suggest that Cullin 3-based ubiquitin proteasome pathway may be used as a promising therapeutic target for NPC.

PMID: 28429677 [PubMed - as supplied by publisher]

from #AlexandrosSfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/2p5ZNiS
via IFTTT