Zebrafish Embryonic Lipidomic Analysis Reveals that the Yolk Cell Is Metabolically Active in Processing Lipid

Publication date: Available online 4 February 2016
Source:Cell Reports
Author(s): Daniel Fraher, Andrew Sanigorski, Natalie A. Mellett, Peter J. Meikle, Andrew J. Sinclair, Yann Gibert
The role of lipids in providing energy and structural cellular components during vertebrate development is poorly understood. To elucidate these roles further, we visualized lipid deposition and examined expression of key lipid-regulating genes during zebrafish embryogenesis. We also conducted a semiquantitative analysis of lipidomic composition using liquid chromatography (LC)-mass spectrometry. Finally, we analyzed processing of boron-dipyrromethene (BODIPY) lipid analogs injected into the yolk using thin layer chromatography. Our data reveal that the most abundant lipids in the embryo are cholesterol, phosphatidylcholine, and triglyceride. Moreover, we demonstrate that lipids are processed within the yolk prior to mobilization to the embryonic body. Our data identify a metabolically active yolk and body resulting in a dynamic lipid composition. This provides a foundation for studying lipid biology during normal or pharmacologically compromised embryogenesis.

Graphical abstract

Teaser

Fraher et al. develop a method to identify lipids separately in the zebrafish yolk and the embryonic body. Lipid usage and content are dynamic in the yolk and embryo during development. The yolk actively processes lipids prior to migration to the body during early embryogenesis.


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