Source:Cell Reports
Author(s): Rajit Rajappa, Anne Gauthier-Kemper, Daniel Böning, Jana Hüve, Jürgen Klingauf
Release site clearance is an important process during synaptic vesicle (SV) recycling. However, little is known about its molecular mechanism. Here we identify self-assembly of exocytosed Synaptobrevin 2 (Syb2) and Synaptophysin 1 (Syp1) by homo- and hetero-oligomerization into clusters as key mechanisms mediating release site clearance for preventing cis-SNARE complex formation at the active zone (AZ). In hippocampal neurons from Syp1 knockout mice, neurons expressing a monomeric Syb2 mutant, or after acute block of the ATPase N-ethylmaleimide-sensitive factor (NSF), responsible for cis-SNARE complex disassembly, we found strong frequency-dependent short-term depression (STD), whereas retrieval of Syb2 by compensatory endocytosis was only affected weakly. Defects in Syb2 endocytosis were stimulus- and frequency-dependent, indicating that Syp1 is not essential for Syb2 retrieval, but for its efficient clearance upstream of endocytosis. Our findings identify an SV protein as a release site clearance factor.
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Rajappa et al. identify self-assembly of exocytosed Synaptobrevin 2 and Synaptophysin 1 by homo- and hetero-oligomerization at endocytic zones as a key mechanism mediating release site clearance. Both prevent cis-SNARE complex formation at the active zone and ensuing short-term depression.from #AlexandrosSfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/1T1Unym
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