Development of one control and one tumor-specific induced pluripotent stem cell line from laryngeal carcinoma patient.

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Development of one control and one tumor-specific induced pluripotent stem cell line from laryngeal carcinoma patient.

Stem Cell Res. 2017 Dec;25:283-285

Authors: Zhang Y, Zhu J, Dai Y, Wang L, Liu R

Abstract
Skin fibroblasts and tumor fibroblasts were extracted from a 64-year old male patient clinically diagnosed with laryngeal carcinoma. Control and tumor specific induced pluripotent stem cells were reprogrammed with 5 reprogramming factors, Klf-4, c-Myc, Oct-4, Sox-2, and Lin-28, using the messenger RNA reprogramming system. The transgene-free iPSC lines showed pluripotency, confirmed by immunofluorescence staining. The iPSC lines also showed normal karyotype, and could form embryoid bodies in vitro and differentiate into the 3 germ layers in vivo. This in vitro cellular model can be used to study the oncogenesis and pathogenesis of laryngeal carcinoma.

PMID: 29246575 [PubMed - in process]

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