Effect of ZEB2 silencing on cisplatin resistance in gastric cancer

OBJECTIVE: To investigate the effect of ZEB2 silencing on cisplatin resistance in gastric cancer.

MATERIALS AND METHODS: The resulting cell line, SGC7901/DDP, was transfected with ZEB2 siRNA, non-specific siRNA, or vehicle control. The effectiveness of ZEB2 silencing was evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot. MTT viability assay was used to determine the cisplatin-sensitivity of cells. Cell apoptosis was measured by flow cytometry.

RESULTS: A significant decrease in ZEB2 in mRNA and protein level was seen in cells transfected with ZEB2 siRNA, compared to that in cells transfected with non-specific siRNA or vehicle. Transfection with ZEB2 siRNA in cisplatin-resistant SGC7901/DDP cells resulted in a significant decrease in cell viability in response to the cisplatin treatment, and cell viability decreased with increasing cisplatin concentrations. A higher apoptotic rate was also seen in cells transfected with ZEB2 siRNA under cisplatin treatment.

CONCLUSIONS: ZEB2 silencing can effectively make gastric cells sensitive to cisplatin treatment in vitro.

L'articolo Effect of ZEB2 silencing on cisplatin resistance in gastric cancer sembra essere il primo su European Review.

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