<span class="paragraphSection"><div class="boxTitle">Abstract</div>Genome-wide association studies (GWASs) have identified multiple single nucleotide polymorphisms (SNPs) associated with colorectal cancer (CRC) susceptibility. However, the elucidation of causal SNPs and the biological mechanisms behind are still limited. In this study, we initially performed systematic bioinformatics analyses on CRC GWAS-identified loci to seek for potential functional SNPs located at transcription factor binding sites (TFBSs), and then a two-stage case-control study comprised of 1353 cases and 1448 controls of Chinese populations and functional analyses were conducted. As a result, only one SNP rs6695837 out of the nine candidate SNPs survived after two-stage analyses by Bonferroni correction. In combined analyses, rs6695837 exhibited significant associations with CRC risk (TT: CC, odds ratio (OR) = 1.31, 95% confidence interval (CI) = 1.06–1.63; dominant model, OR = 1.21, 95% CI = 1.03–1.43; additive model, OR = 1.15, 95% CI = 1.03–1.28). Functional annotations by RegulomeDB and rSNPBase indicated its biological role and dual-luciferase reporter assays revealed a significant increase in luciferase expression for the reconstructed plasmid with rs6695837T allele, compared with the one with C allele (<span style="font-style:italic;">P</span><sub>SW480</sub> = 0.0002, <span style="font-style:italic;">P</span><sub>Lovo</sub> = 0.0003). Further gene expression analyses demonstrated significantly higher expression of <span style="font-style:italic;">LAMC1</span> gene in CRC tumor tissues than that in adjacent non-cancerous tissues (<span style="font-style:italic;">P</span> = 0.0004). These findings strongly suggest that the functional SNP located at TFBSs, rs6695837 might contribute to CRC susceptibility, and the exact biological mechanism awaits further research.</span>
from #AlexandrosSfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/2kWT4lS
via IFTTT
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Δημοφιλείς αναρτήσεις
-
from #AlexandrosSfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/2jCvX3K via IFTTT
-
Abstract Purpose of Review Our goal is to summarize the airway disease literature since September 11, 2001 (9/11), focusing on studies pub...
-
Treatment effectiveness holds considerable importance in the association between service quality and satisfaction in medical service studies...
-
Vol.25 No.2 from #AlexandrosSfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/1P7bHxT via IFTTT
-
Publication date: Available online 16 January 2017 Source: International Journal of Pediatric Otorhinolaryngology Author(s): Kaveh Karimn...
-
Understanding Head And Neck Cancers - The Southeast Sun ... The Southeast Sun (NAPSI)—According to the Centers for Disease Control an...
-
Abstract Background Henoch–Schönlein purpura is the most common vasculitis in children. Its long-term prognosis depends on renal involve...
-
Publication date: June 2017 Source: International Journal of Biological Macromolecules, Volume 99 Author(s): Oliviero Carugo The positio...
-
Publication date: 21 February 2017 Source: Cell Reports, Volume 18, Issue 8 Author(s): Arielle Glatman Zaretsky, Christoph Konradt, Fabien...
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου