Subclinical Alteration of the Cervical-Vaginal Microbiome in Women with Idiopathic Infertility

ABSTRACT

Biomarkers have a wide application in research and clinic, they help to choose the correct treatment for diseases. Recent studies, addressing the vaginal microbiome using next generation sequencing (NGS), reported the involvement of bacterial species in infertility. We compared the vaginal microbiome of idiopathic infertility diagnosed women with that of healthy, non-idiopathic infertile and bacterial vaginosis affected women, to identify bacterial species suitable as biomarkers. Information on microorganisms was obtained from the V3-16S rDNA sequencing of cervical-vaginal fluids of 96 women using the Ion Torrent platform. Data were processed with QIIME and classified against the Vaginal 16S rDNA Reference Database. The analysis revealed a significant beta-diversity variation (p < 0.001) between the 4 groups included in the study: women affected by idiopathic infertility, women not affected by idiopathic infertility, women with bacterial vaginosis, and healthy control women. Lactobacilli iners, crispatus, and gasseri distinguished Idiopathic from women of the other groups. Lactobacilli showed a different distribution between women with idiopathic infertility and women with a known cause of infertility. Our results suggest that women with repeated failed in vitro fertilization cycles may benefit from microbial screening by NGS. Due to its high throughput, NGS screening could increase the accuracy of available tools for the diagnosis of infertility and improve therapeutic protocols. This article is protected by copyright. All rights reserved

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