20-Hydroxyecdysone from Dacrycarpus imbricatus bark inhibits the proliferation of acute myeloid leukemia cells

Publication date: Available online 21 January 2017
Source:Asian Pacific Journal of Tropical Medicine
Author(s): Trinh Thi Thuy, Nguyen Thanh Tam, Nguyen Thi Hoang Anh, Dang Viet Hau, Dinh Thi Phong, Le Quoc Thang, Sabrina Adorisio, Tran Van Sung, Domenico V. Delfino
ObjectiveTo investigate the anti-proliferative effects of 20-hydroxyecdysone isolated from the bark of Dacrycarpus imbricatus (Blume) de Laub.MethodsColumn chromatography was used for isolation of compounds from plant material. The structure of the isolated compound was identified by mass spectrometry and nuclear magnetic resonance techniques, including HSQC, HMBC, NOE-difference experiments. The isolated compound was tested for its anti-proliferative activity in acute myeloid leukemia (AML) and OCI-AML cells.ResultsCompound 1 was isolated from the ethyl acetate fraction of Dacrycarpus imbricatus barks by column chromatography. Its chemical structure was identified as 20-hydroxyecdysone (20HE), a cholestane-type ecdysteroid, by a combination of mass spectrometry and nuclear magnetic resonance spectrometric analyses. Our goal was to test the anti-proliferative activity of 20HE using the OCI-AML cell line. 20HE significantly decreased OCI cell number at a concentration of 1 mg/mL, whereas lower concentrations were ineffective. Moreover, this decrease was due to partial blockage of the G1/S phase of the cell cycle, with a reduction of cells in the G2M phase, not due to increased apoptosis.ConclusionsThis indicates that 20HE significantly decreases the number of cells in the G1/S phase of the cell cycle in human AML cells. This is the first time that the anti-proliferative activity of 20HE against a human tumor cell line has been reported.



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