Stem cell-based therapy is a promising treatment strategy for neurodegenerative diseases such as Alzheimer’s disease (AD). However, the mechanism underlying the maintenance of renewal and replacement capabilities of endogenous progenitor cells or engrafted stem cells in a pathological environment remains elusive. To investigate the effect of astragaloside IV (ASI) on the proliferation and differentiation of the engrafted neural stem cells (NSCs), we cultured NSCs from the hippocampus of E14 rat embryos, treated the cells with ASI, and then transplanted the cells into the hippocampus of rat AD models. In vitro experimentation showed that 10−5 M ASI induced NSCs to differentiate into β-tubulin III+ and GFAP+ cells. NSCs transplantation into rat AD models resulted in improvements in learning and memory, especially in the ASI-treated groups. ASI treatment resulted in an increase in the number of β-tubulin III+ cells in the hippocampus. Further investigation showed that ASI inhibited PS1 expression in vitro and in vivo. The high-dose ASI downregulated the Notch intracellular domain, whereas the low-dose ASI increased Notch-1 and NICD. In conclusion, ASI treatment resulted in improvements in learning and memory of AD models by promoting NSC proliferation and differentiation partly through the Notch signal pathway.
from #AlexandrosSfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/1TCuCF6
via IFTTT
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Δημοφιλείς αναρτήσεις
-
The HiFocus Mid-Scala (MS) electrode array has recently been introduced onto the market. This precurved design with a targeted mid-scalar in...
-
Abstract Background Albumin is a protein colloidal solution with limited availability and high cost. It should be used in such approved i...
-
Publication date: Available online 6 November 2017 Source: Journal of Dairy Science Author(s): Lorenzo E. Hernández-Castellano, Ranya Özçe...
-
Abstract Cigarette smoking is associated with an increased risk of developing mucinous ovarian tumours but whether it is associated with o...
-
<span class="paragraphSection"><div class="boxTitle">Abstract</div><div class="boxTitle"...
-
by Jiansong Chen, Borong Lin, Qiuling Huang, Lili Hu, Kan Zhuo, Jinling Liao Plant pathogen effectors can recruit the host post-translatio...
-
CDC and health officials from Wisconsin and Illinois are conducting an investigation of Seoul virus infections among pet rats and persons ex...
-
Bacteriological confirmed active case detection remains the corner stone for diagnosing tuberculosis. Non-radiometric liquid culture system ...
-
Atooshe Rohani, Shahram Kargar, Afsoon Fazlinejad, Fereshte Ghaderi, Vida Vakili, Homa Falsoleiman, Ramin Khamene Bagheri Annals of Cardi...
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου