Expression of cyclin d1 correlates with p27KIP1 and regulates the degree of oral dysplasia and squamous cell carcinoma differentiation.

Publication date: Available online 1 February 2018
Source:Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Author(s): Guangzhao Guan, Mahmoud M. Bakr, Norman Firth, Robert M. Love
Objectives.The aim of this study was to show an association or link between cyclin D1 and P27^KIP1 protein expression and dysplastic changes or progression.Study design.Oral mucosal biopsies with a diagnosis of non-neoplastic tissue (Gingivitis) (n=10), mild to moderate oral epithelial dysplasia (n=12) and oral squamous cell carcinoma (n=11) were evaluated by using immunohistochemistry. Scanning software was used to determine cyclin D1 and p27^KIP1 intensity of expression, location and pattern.Results.A significant increase in expression of cyclin D1 and a decrease in expression of p27^KIP1 proteins were observed in oral epithelial dysplasia and less differentiated OSCC. There was a more diffuse distribution of cyclin D1 protein expression extending from the basal cell layer into the prickle cell layers in epithelial dysplasia, and extending within all epithelial layers in OSCC. Cases of oral epithelial dysplasia showed moderate infrequent expression of p27^KIP1. There were no p27^KIP1 positive cells in OSCC. The percentage of cells with both nuclear and cytoplasmic cyclin D1 staining was higher in oral squamous cell carcinoma specimens than control groups and oral epithelial dysplasia.Conclusions.The expression of both cyclin D1 and p27^KIP1 correlate with the grade of oral epithelial dysplasia and degree of OSCC differentiation. The results obtained will be verified through a basic follow up of the cases to determine the prognosis/progression of oral dysplasia.



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