Epicutaneous exposure to allergens through the skin is considered to be an important route of sensitization.1,2 Allergen sources, such as mites, insects, fungi, and pollen, contain proteases, which are frequently allergens themselves.3 Recent studies using murine models of sensitization via skin4 or airways5-7 revealed that the protease activity of a model protease allergen, papain, is essential to the induction of inflammation at the sensitization sites and serum IgE/IgG1 responses. However, the role of allergen protease activity in effector-phase responses against low-dose antigen encounters after epicutaneous sensitization currently remains unknown.
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