Increased infiltration of CD11c+/CD123+ dendritic cell subsets and upregulation of TLR/IFN-α signaling participate in pathogenesis of oral lichen planus

Publication date: Available online 18 December 2017
Source:Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Author(s): Yufeng Wang, Shu Shang, Qianqian Sun, Junjun Chen, Guanhuan Du, Hong Nie, Xiaozhe Han, Guoyao Tang
ObjectiveInvestigation of dendritic cell (DC) subsets and expression patterns of Toll-like receptors (TLRs) was conducted to understand the pathogenesis in oral lichen planus (OLP).Study DesignBlood, OLP lesion and control samples were collected. Four DC subsets (CD11c⁺CD123^-myeloid DC-mDC1, CD141⁺myeloid DC-mDC2, CD11c^-CD123⁺plasmacytoid DC-pDC and CD1a⁺CD207⁺Langerhans cells-LC) were investigated via flow cytometry (FCM) and immunohistochemical (IHC) staining. Expression patterns of TLRs and their downstream molecules were analyzed via qRT-PCR and IHC in situ.ResultsThirty-two samples were collected (9 controls and 23 OLP patients). FCM results showed the percentages of LC, mDC1, mDC2 and pDC in situ were 0.0119±0.0251%, 0.0064±0.0134%, 0.0005±0.0011%, and 0.0022±0.0019% in control mucosa, respectively. The mDC1 (0.0300±0.0276%) and pDC (0.0204±0.0186%) subsets were significantly increased in OLP lesions (p<0.01). No marked differences were evident, when comparing all four DC subsets from blood, between control and OLP groups. Significant upregulation of TLR7, TLR8 and TLR9 were disclosed in OLP (p<0.01), along with their downstream interferon-α(IFN-α) signaling molecules(IRF7 and IFN-α, p<0.01).ConclusionOur findings of increased infiltration of pDC and mDC1, along with upregulation of TLR/IFN-α signaling, provide valuable information for further understanding the immunity in OLP.



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