Comparative Liquid Chromatographic Study for Concurrent Determination of Canagliflozin and Metformin in Combined Tablets

New HPLC-UV method (method A), for simultaneous determination of metformin (MET) and canagliflozin (CANA), was developed and compared to another novel UPLC-UV method (method B) in their tablet combination. Concerning method A, isocratic separation was done by C18 column (100 mm 2.1 mm, 3 m) using methanol and 0.03 M phosphate buffer (, ) at pH 3.2 as a mobile phase. Meanwhile, chromatographic separation in method B was achieved via Hypersil® gold (50 mm 2.1 mm, 1.9 m). Mobile phase was methanol and 0.03 M phosphate buffer at ratio of   . In both, detection was done at wavelength of 240 nm. Method A showed satisfactory linearity results over 1–50 gmL−1 and 0.5–100 gmL−1, while method B linearity was at 0.1–50 gmL−1 and 0.25–100 gmL−1 for CANA and MET, respectively. In terms of accuracy and precision, method A accuracy was and , while method B gave accuracy of and for CANA and MET, respectively. For precision, the % RSD was found to be less than 2% for three concentrations analyzed three times. The two methods are convenient for quality laboratories, yet the UPLC method offered the advantage of shorter run times and higher sensitivity.

from #AlexandrosSfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/2khU6y4
via IFTTT