Investigating the Effect of Fibulin-1 on the Differentiation of Human Nasal Inferior Turbinate-Derived Mesenchymal Stem Cells into Osteoblasts.

Investigating the Effect of Fibulin-1 on the Differentiation of Human Nasal Inferior Turbinate-Derived Mesenchymal Stem Cells into Osteoblasts.

J Biomed Mater Res A. 2017 Apr 26;:

Authors: Pham LBH, Yoo YR, Park SH, Back SA, Kim SW, Bjørge I, Jang JH

Abstract
Many extracellular matrix proteins have positive influences on the adhesion, proliferation and differentiation of stem cells into specific cell linages. Fibulin-1 (FBLN1), a member of a growing family of extracellular glycoproteins, contributes to the structure of the extracellular matrix. Here, we investigated the effect of FBLN1 on the ability of human nasal inferior turbinate-derived mesenchymal stem cells (hTMSCs) to undergo osteogenic differentiation. After we generated recombinant FBLN1, the characteristics of FBLN1-treated hTMSCs were evaluated using MTT assay, ALP and mineralization activities, and quantitative real-time PCR. FBLN1 significantly enhanced the adhesion activity (p < 0.001) and proliferation of hTMSCs (p < 0.05). The ALP and mineralization activities of cells were dramatically increased (p < 0.01) after 9 days and 12 days of FBLN1 treatment, respectively.This, indicated the ability of FBLN1 to induce hTMSCs to differentiate into osteoblasts. Furthermore, increasing the mRNA levels of osteogenic marker genes, such as a transcriptional coactivator with a PDZ-binding motif (TAZ), alkaline phosphatase (ALP), collagen type I (Col I), and osteocalcin (OCN), improved bone repair and regeneration. This article is protected by copyright. All rights reserved.

PMID: 28445604 [PubMed - as supplied by publisher]

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