Characterization of the selective alkylation site in hemoglobin A by dihydroartemisinin with tandem mass spectrometry

Publication date: June 2017
Source:International Journal of Biological Macromolecules, Volume 99
Author(s): Khomsan Tiensomjitr, Samran Prabpai, Palangpon Kongsaeree
The reaction between the antimalarial drug dihydroartemisinin (DHA) and hemoglobin A (HbA) was investigated in vitro. A fluorescein-tagged artemisinin analog reacted with HbA and fluorescent HbA-drug adducts could be visualized on SDS-PAGE to confirm stable covalent reaction adducts and necessity of the endoperoxide moiety and Fe(II). Mass spectrometric analyses revealed that DHA favourably alkylated protein part rather than heme and the modification site was identified to be at Tyr35 of the beta globin chain.

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