Publication date: Available online 11 February 2017
Source:Biochimie
Author(s): Vanir Reis Pinto-Junior, Vinicius Jose Silva Osterne, Mayara Queiroz Santiago, Claudia Figueiredo Lossio, Celso Shiniti Nagano, Cintia Renata Costa Rocha, Jessica Catarine Frutuoso Nascimento, Francisco Lucas Faustino Nascimento, Ivanice Bezerra Silva, Antonia Simoni Oliveira, Jorge Luis Almeida Correia, Rodrigo Bainy Leal, Ana Maria Sampaio Assreuy, Benildo Sousa Cavada, Kyria Santiago Nascimento
Lectins are proteins, or glycoproteins, capable of reversibly binding to specific mono- or oligosaccharides via a noncatalytic domain. The Diocleinae subtribe presents lectins with high structural similarity, but different effects based on biological activity assays. This variability results from small structural differences. Therefore, in this context, the present study aimed to perform a structural analysis of the lectin from Dioclea lasiophylla Mart. ex Benth seeds (DlyL) and evaluate its inflammatory effect. To accomplish this, DlyL was purified in a single step by affinity chromatography on Sephadex® G-50 matrix. DlyL primary structure was determined through a combination of tandem mass spectrometry and DNA sequencing. DlyL showed high similarity with other species from the same genus. Its theoretical three-dimensional structure was predicted by homology modelling, and the protein was subjected to ligand screening with monosaccharides, oligosaccharides and complex N-glycans by molecular docking. Stability and binding of the lectin with α-methyl-d-mannoside were assessed by molecular dynamics. DlyL showed acute inflammatory response with hypernociceptive effect in the paw edema model, possibly by interaction with glycans present at the cell surface.
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