Background. Growth arrest-specific (Gas) 6 is one of the endogenous ligands of TAM receptors (Tyro3, Axl, and Mertk), and its role as an immune modulator has been recently emphasized. Naturally occurring CD4+CD25+ regulatory T cells (Tregs) are essential for the active suppression of autoimmunity. The present study was designed to investigate whether Tregs express TAM receptors and the potential role of Gas6-TAM signal in regulating the suppressive function of Tregs. Methods. The protein and mRNA levels of TAM receptors were determined by using Western blot, immunofluorescence, flow cytometry, and RT-PCR. Then, TAM receptors were silenced using targeted siRNA or blocked with specific antibody. The suppressive function of Tregs was assessed by using a CFSE-based T cell proliferation assay. Flow cytometry was used to determine the expression of Foxp3 and CTLA4 whereas cytokines secretion levels were measured by ELISA assay. Results. Tregs express both Axl and Mertk receptors. Gas6 increases the suppressive function of Tregs in vitro and in mice. Both Foxp3 and CTLA-4 expression on Tregs are enhanced after Gas6 stimulation. Gas6 enhances the suppressive activity of Tregs mainly through Axl receptor. Conclusion. Gas6 has a direct effect on the functions of CD4+CD25+Tregs mainly through its interaction with Axl receptor.
from #AlexandrosSfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/2kmc9OL
via IFTTT
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Δημοφιλείς αναρτήσεις
-
When you make pizza this good you are allowed to brag about it. Come try a square of the best pizza in Erie. Ohhh yeah, we have ice cream no...
-
British Journal of Oral and Maxillofacial Surgery... Stem Cells and DevelopmentMesenchymal Stem/Stroma... [ASAP] Self-Assembly of Giant Mo...
-
A new book examines an epic study in behavioural genetics, but forgets to include the detail – or explain why so many domesticated creatures...
-
Publication date: Available online 25 January 2017 Source: Free Radical Biology and Medicine Author(s): Ana Cipak Gasparovic, Lidija Milk...
-
Abstract ᅟ http://ift.tt/2pGNeby
-
from #AlexandrosSfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/2o6Gupp via IFTTT
-
The efficacy and optimization of poststroke physical therapy paradigms is challenged in part by a lack of objective tools available to resea...
-
Related Articles Exome Sequencing of Oral Squamous Cell Carcinoma Reveals Molecular Subgroups and Novel Therapeutic Opportunities. Th...
-
Summary Background Autologous non-cultured cell suspension transplantation is an effective treatment for repigmentation in segmental vit...
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου