In vivo Modelling of the Pathogenic Effect of Copper Transporter Mutations that cause Menkes and Wilson Disease, Motor Neuropathy and Susceptibility to Alzheimer's Disease [Metabolism]

Copper is an essential biometal and several inherited diseases are directly associated with a disruption to normal copper homeostasis. The best characterised are the copper deficiency and toxicity disorders Menkes and Wilson disease, caused by mutations in the p-Type Cu-ATPase genes ATP7A and ATP7B respectively. Missense mutations in the C-terminal portion of ATP7A have also been shown to cause Distal Motor Neuropathy while polymorphisms in ATP7B are associated with increased risk of Alzheimer's disease. We have generated a single, in vivo model for studying multiple pathogenic mutations in ATP7 proteins, using Drosophila melanogaster, which has a single orthologue of ATP7A and ATP7B. Four pathogenic ATP7A mutations and two ATP7B mutations were introduced into a genomic ATP7 rescue construct containing an in-frame C-terminal GFP tag. Analysis of the wild type ATP7:GFP transgene confirmed that ATP7 is expressed at the basolateral membrane of larval midgut copper cells and that the transgene can rescue a normally early-lethal ATP7 deletion allele to adulthood. Analysis of the gATP7:GFP transgenes containing pathogenic mutations showed that the function of ATP7 was affected, to varying degrees, by all six of the mutations investigated in this study. Of particular interest, the ATP7BK832R Alzheimer's disease susceptibility allele was found, for the first time, to be a loss of function allele. This in vivo system allows us to assess the severity of individual ATP7A/B mutations in an invariant genetic background and has the potential to be used to screen for therapeutic compounds able to restore function to faulty copper transport proteins.

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