Thin-Film Transistor-Based Biosensors for Determining Stoichiometry of Biochemical Reactions

by Yi-Wen Wang, Ting-Yang Chen, Tsung-Han Yang, Cheng-Chung Chang, Tsung-Lin Yang, Yu-Hwa Lo, Jian-Jang Huang

The enzyme kinetic in a biochemical reaction is critical to scientific research and drug discovery but can hardly be determined experimentally from enzyme assays. In this work, a charge-current transducer (a transistor) is proposed to evaluate the status of biochemical reaction by monitoring the electrical charge changes. Using the malate-aspartate shuttle as an example, a thin-film transistor (TFT)-based biosensor with an extended gold pad is demonstrated to detect the biochemical reaction between NADH and NAD⁺. The drain current change indicates the status of chemical equilibrium and stoichiometry.

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