Scenedesmus vacuolatus cultures for possible combined laccase-like phenoloxidase activity and biodiesel production

Abstract

A key aspect of the industrial development of microalgal production processes is the excessive cost of biomass production. A solution is a combination of biodiesel production and wastewater treatment. The microalga Scenedesmus has a high lipid content and a potential extracellular phenoloxidase activity, which could improve the phycoremediation of phenolic pollutants. In this work, the most suitable growth conditions to obtain this twofold aim were analyzed. First, different strains of Scenedesmus vacuolatus microalga were tested at different pH, salinity and CO₂ concentration in the gas phase. The two most promising strains were then cultivated in autotrophic and heterotrophic conditions, and were investigated in terms of efficient nitrogen removal, fatty acid profile and maximized extracellular phenoloxidase activity in the medium. The results showed two extreme conditions: (1) biomass productivity doubled when photobioreactors were sparged with 5% CO₂ supplemented air with respect to cultures sparged with air (the steady state values of strain 53 were 0.138 g L⁻¹ day⁻¹ in the presence of air, and 0.243 in the presence of CO₂ addition), and N-starvation under 5% CO₂enhanced the transesterified fraction of lipids (strain 53 FAME fraction in the presence of N-starvation was 33%, in the presence of nitrogen FAME fraction was 22%); (2) phenoloxidase activity was completely suppressed by presence of 5% CO₂ in the gas phase (strain 53 0.21 U mL⁻¹), indicating clear catabolite repression for the induction of this enzyme in the algal metabolism.

from #AlexandrosSfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/2j6Y1u4
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