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Transcription factor ΔFosB acts within the nucleus of the solitary tract to increase mean arterial pressure during exposures to intermittent hypoxia.
Am J Physiol Heart Circ Physiol. 2017 Nov 03;:ajpheart.00268.2017
Authors: Wu Q, Cunningham JT, Mifflin SW
Abstract
ΔFosB is a member of the AP-1 family of transcription factors. ΔFosB has low constitutive expression in the central nervous system and is induced following exposure of rodents to intermittent hypoxia (IH), a model of the arterial hypoxemia that accompanies sleep apnea. We hypothesize ΔFosB in the nucleus of solitary tract (NTS) contributes to increased mean arterial pressure (MAP) during IH. The NTS of 11 male Sprague Dawley rats was injected (3 sites, 100nl per site) with a dominant-negative against ΔFosB (ΔJunD) in an adeno-associated viral vector (AAV)-green fluorescent protein (GFP) reporter. The NTS of 10 rats was injected with AAV-GFP as sham controls. Two weeks after NTS injections rats were exposed to IH for 8h/day, 7 days and MAP recorded using telemetry. In the sham group 7 days IH increased MAP from 99.8 ± 1.1 mmHg to 107.3 ± 0.5 mmHg in the day and from 104.4 ± 1.1 mmHg to 109.8 ± 0.6 in the night. In the group receiving ΔJunD, IH increased MAP during the day from 95.9 ± 1.7 mmHg to 101.3 ± 0.4 mmHg; during the dark MAP increased from 100.9 ± 1.7 mmHg to 102.8 ± 0.5 mmHg (both significantly lower than sham p<0.05). Following injection of the dominant-negative construct in the NTS, IH-induced ΔFosB immunoreactivity was decreased in paraventricular nucleus (p < 0.05), however no change was observed in rostral ventrolateral medulla. These data indicate that ΔFosB within the NTS contributes to the increase in MAP induced by IH exposure.
PMID: 29101166 [PubMed - as supplied by publisher]
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