Molecular cloning and characterization of the SIFamide precursor and receptor in a hymenopteran insect, Bombus terrestris.

Molecular cloning and characterization of the SIFamide precursor and receptor in a hymenopteran insect, Bombus terrestris.

Gen Comp Endocrinol. 2017 Nov 07;:

Authors: Lismont E, Mortelmans N, Verlinden H, Broeck JV

Abstract
SIFamides (SIFa) are a family of neuropeptides that are highly conserved among arthropods. In insects, this peptide is mainly expressed in four medial interneurons in the pars intercerebralis and affects sexual behavior, sleep regulation and pupal mortality. Furthermore, an influence on the hatching rate has been observed. The first SIFa receptor (SIFR) was pharmacologically characterized in Drosophila melanogaster and is homologous to the vertebrate gonadotropin-inhibitory hormone (GnIH) receptor (NPFFR). In this study, we pharmacologically characterized the SIFR of the buff-tailed bumblebee Bombus terrestris. We demonstrated an intracellular increase in calcium ions and cyclic AMP (cAMP) upon ligand binding with an EC50 value in the picomolar and nanomolar range, respectively. In addition, we studied the agonistic properties of a range of related and modified peptides. By means of quantitative real time PCR (qPCR), we examined the relative transcript levels of Bomte-SIFa and Bomte-SIFR in a variety of tissues.

PMID: 29127004 [PubMed - as supplied by publisher]

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