In several recent studies, proteomics analyses suggest that increase of ubiquinol-cytochrome c reductase core protein 1 (UQCRC1) is cardio-protective. However, direct evidence for this effect has not yet been obtained. Thus, the current study aimed to determine this effect and the mechanism underlying this effect. The results showed that overexpression of UQCRC1 protected H9c2 cardiac cells against in vitro simulated ischemia-reperfusion by maintaining mitochondrial membrane potential and suppressing the expression of caspase-3. These protective effects were significantly enhanced by exogenous Zn2+ but completely abolished by Zn2+-selective chelator TPEN. Furthermore, the upregulation of UQCRC1 reduced the concentration of free Zn2+ in mitochondria, whereas the downregulation of UQCRC1 increased the concentration of free Zn2+ in mitochondria. In conclusion, the overexpression of UQCRC1 can protect H9c2 cardiac cells against simulated ischemia/reperfusion, and this cardio-protective effect is likely mediated by zinc binding.
from #AlexandrosSfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/2szfBtK
via IFTTT
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Δημοφιλείς αναρτήσεις
-
Publication date: October 2017 Source: International Journal of Biological Macromolecules, Volume 103 Author(s): Mingan Yu, Duqiang Liu, ...
-
Abstract Endurance exercise generates CO 2 via aerobic metabolism; however, its role remains unclear. Exogenous CO 2 by transcutaneous d...
-
FS Teni, AS Surur, A Hailemariam, A Aye, G Mitiku, AE Gurmu, B Tessema Annals of Medical and Health Sciences Research 2015 5(6):454-460 ...
-
Publication date: October 2017 Source: Free Radical Biology and Medicine, Volume 111 Author(s): Mengqing Xiao, Huiqin Zhong, Lin Xia, Yong...
-
Abstract Background Studies exploring the association between physical activity, screen time and sleep and pain usually focus on a limit...
-
RT @caonline : The role of extranodal extension in staging head and neck cancers: http://ift.tt/2qV1As1 #hncsm https://t.co/MxlLX4Olo6 f...
-
One third of adult patients with orthognathic surgery of a prognathic or retrognathic mandible show relapse. The sagittal split osteotomy of...
-
Abstract This paper analyzes the most and least used upper limb aquatic equipment for hydrotherapy/hydro kinesiology specialists in the tr...
-
Abstract Wheat germ has an important enzymatic activity, being lipases the enzymes which cause the highest impact in the reduction of shelf...
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου