Antigen-specific immunoglobulin variable region sequencing measures humoral immune response to vaccination in the equine neonate

by Rebecca L. Tallmadge, Steven C. Miller, Stephen A. Parry, Maria Julia B. Felippe

The value of prophylactic neonatal vaccination is challenged by the interference of passively transferred maternal antibodies and immune competence at birth. Taken our previous studies on equine B cell ontogeny, we hypothesized that the equine neonate generates a diverse immunoglobulin repertoire in response to vaccination, independently of circulating maternal antibodies. In this study, equine neonates were vaccinated with 3 doses of keyhole limpet hemocyanin (KLH) or equine influenza vaccine, and humoral immune responses were assessed using antigen-specific serum antibodies and B cell Ig variable region sequencing. An increase (pin vitro immunogen stimulation indicated limited lymphocyte population expansion in response to vaccine during the study period.

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