Substrate recognition by the Cdh1 destruction box receptor is a general requirement for APC/CCdh1-mediated proteolysis. [Additions and Corrections]

VOLUME 291 (2016) PAGES 15564–15574There were several errors in this article. In the Cdc5 experiment in Fig. 3A, the anti-G6PD immunoblot image from the Spo13 experiment in Fig. 3B was accidentally duplicated and flipped horizontally. Also in Fig. 3A, the experiment labeled Nrm1 actually shows the results from the Mps1 experiment. In Fig. 4C, the Spo12 stability profile in the wild-type CDH1 strain is identical to that shown in Fig. 3B. In Fig. 4, B and C, a black bar is now used to indicate excision of lanes from the same exposure of the same immunoblot. A white vertical bar is still used to indicate comparison of different exposures where applicable. In Fig. 5A, the original Cdc5 panel showed a G6PD immunoblot image derived from the Kip1 panel shown in Fig. 4C. Although the images were not identical, an examination of the original data revealed that the actual Kip1 membrane was mistakenly used again to generate the G6PD load control image shown for the Cdc5 experiment. As a result, no appropriate load control data exists for the Cdc5 assay in the ABBA motif receptor mutant strain, and this panel has been removed from Fig. 5A. Additionally, in Fig. 5, only the positive results shown in Fig. 5B had three independent experiments. The negative results depicted in Fig. 5A were obtained in either one or two independent experiments. These errors have now been corrected and do not affect the results or conclusions of this work.jbc;292/12/5125/T1T1T1TABLE 1Summary of APC/CCdh1 substrates and resultsSubstrateaFunctional...

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